Culture of sheep poxvirus in a 3D Vero cells culture model

Abstract

In the article the production of a three-dimensional culture of Vero cells using the methods of “hanging drop” and roller cultivation was described. The resulting spheroids remain viable for more than four days. For the formation of large spheroids with good proliferative activity, the inoculum concentration should be 200000 cells/ml. The additional study of the cell proliferation index in spheroids showed that cell growth was 3.5 times on the second day. A comparative study of the biological activity of the sheep poxvirus in 3D cell culture was carried out in comparison with traditional2D cultivation. After infection of the spheroids with sheep poxvirus in the first passage, the virus titer was 5.33±0.04 lg TCID₅₀/ml. At the second and third passages, the biological activity of the virus increased to 6.33±0.04 and 6.41±0.04 lg TCID₅₀/ml, respectively. Considering these data, three-dimensional culture allows for obtaining much cell biomass with high biological activity compared to the traditional culture method.