Histochemical method for fluorescent staining of Zn +2 -ions in glands

Authors

  • A.A. Kikimbaeva
  • Z.T. Kystaubaeva
  • G.M. Tykezhanova
  • L.K. Bystrevska
  • A.G. Abdraimova-Meyramova
  • Ye.M. Laryushina
  • S.B. Zhautikova
  • V.N. Beskov
  • G.O. Zhuzbaeva
  • A.R. Alina
  • S.S. Turzhanova
  • G.K. Turlibekova

DOI:

https://doi.org/10.31489/2015bmg2/51-55

Keywords:

B-cells, Prostate, Salivary glands, 8-para(toluenesulphonylamino)quinolone, Zn +2 -ions

Abstract

It is showed by authors that using of 8-para(toluenesulphonylamino)quinoline — a derivative of 8-oxyquino-lin result histochemical revealing by using of fluorescent microscopy of Zn+2-ions in cells of tissue of pros-tate, in pancreatic B-cells and in salivary glands contains a large amounts of ions. This method is high sensitive and high specific for revealing of Zn+2-ions and there are only one step of staining procedures. Mean-while it is possible to use only fresh frozen sections of tissues for investigation within short time limited by 15–20 min.

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Published

2015-06-30

Issue

2015 (78) 2

Section

Articles