Using of cryoprotectors for low-temperature storage of seed material of Thymus rasitatus
DOI:
https://doi.org/10.31489/2017bmg2/25-30Keywords:
Thymus rasitatus, cryoprotector, cryoconservation, DMSO, glycerin, germination, germinative energy, endemicsAbstract
In article influence of various cryoprotectors onpreserving viability of the seed material of Thymus rasitatus is studied and described. As a result of the conducted researches it is established that for Thymus rasitatus seeds the best storage conditions allowing to keep the highest rates of viability is use of a plastic container, a cryoprotector — 5% DMSO and slow defrosting at the room temperature. At the same time increase in level of energy of germination and viability by 15% and 10,7% in comparison with control is observed. After analyzing dynamics of seed germination of the researched species it was established that the seeds which were previously processed by glycerin and 5% DMSO with the subsequent cryopreservation sprout quicker and
phases of development pass rather.