Extraction of betulin — natural triterpenoid from Kirgyz birch Betula kirghisorum, an endemic plant of the Republic of Kazakhstan
DOI:
https://doi.org/10.31489/2019bmg1/14-18Keywords:
бетулин, береза киргизская, береста, экстракция, изопропиловый спирт, фильтрат, тонкослойная хроматография, пластиныSilufol, элюент, проявитель, ИК-спектроскопия, ВЭЖХ, betulin, Kyrgyz birch, birch bark, extraction, isopropyl alcohol, filtrate, thin layer chromatog-raphy, Silufol plates, eluent, developer, IR spectroscopy, HPLCAbstract
On the territory of the Republic of Kazakhstan there are 15 species of birch trees, including 4 endemic spe-cies. This plant is a low tree with a reddish bark and a loose crown. On the territory of the Kent forestry of the
Karkaraly State National Natural Park, a Kyrgyz birch bark was collected. Birch bark was crushed and dried
to constant weight. From birch bark of the Kyrgyz with extraction method followed by recrystallization from
isopropyl alcohol was isolated betulin. The melting point of the isolated substance was 243 ºC. Betulin was
identified by thin layer chromatography and compared with a standard sample. The substance was analyzed
by infrared spectroscopy methods in KBr tablets and high performance liquid chromatography (HPLC). In the
Fourier spectrometer FSM-1201, the characteristic frequencies of the absorption bands were used to deter-mine the presence of various groups of atoms and bonds characteristic of betulin in the molecule. HPLC was
used to study a sample using a Shimadzu LC-20 Prominence liquid chromatograph. Selected conditions for
the qualitative and quantitative determination of betulin
