Antibacterial activity of ultrasonic extracts of Salvia stepposa growing in Kazakhstan

Abstract

This article presents data on the studies on antibacterial activity of ultrasonic extracts of Salvia stepposa (steppe sage) growing wild on the territory of the Republic of Kazakhstan. Screening for the antibacterial activity of the presented samples was carried out by the discdiffusion method against strains of gram-positive bacteria Staphylococus aureus, Bacillus subtilis, gram-negative strains Escherichia coliand the yeast Candida albicans. The results showed that in relation to Staphylococus aureusthe most pronounced antibacterial activity with growth inhibition zones 35 ± 1 mm is possessed by 30 % ultrasonic extract of steppe sage flowers, in relation to Bacillus subtilis49 ± 1 mm — 40 % ultrasonic extract of steppe sage leaves, for Escherichia coli24 ± 1 mm — 70 % ultrasonic extract of steppe sage leaves. This study demonstrated that, 30 % ultrasonic extract of flowers and 90 % ultrasonic extract of flowers and leaves of steppe sage showed weak activity against yeast fungus Candida albicanswith growth inhibition zones 12 ± 1 mm, 12 ± 1 mm and 11 mm, respectively. As a result, out of 8 extracts isolated by ultrasonic activation only 2 extracts showed antibacteri-al activity against 4 studied bacteria. Additionally, it was found that the use of ultrasonic extraction can re-duce the extraction time of biologically active substances in 8–9 times compared to conventional extraction methods.