The effect of growth regulators on the multiplication of Crataegus sanguinea in vitro

Abstract

This study focused on examining how plant growth regulators affect shoot proliferation on Crataegus sanguinea. Shoot multiplication treatments included benzylaminopurine (6-BAP) at different concentrations (0.25, 0.5, and 0.75 mg/l) combined with constant 0.5 mg/l gibberellic acid (GA3) and 0.01 mg/l Indole-3-butyric acid (IBA), in Quoirin & Lepoivre (QL) nutrient medium. The results showed that the highest number of shoots per explant (5.23) was achieved in a medium supplemented with 0.5 mg/l 6-BAP plus 0.5 mg/l GA3 and 0.01 mg/l IBA, while the greatest shoot length (12,77 cm) was obtained with 0.75 mg/l 6-BAP plus 0.5 mg/l GA3, and 0.01 mg/l IBA 35 days after transplantation. This study presents an efficient protocol for in vitro multiplication of Crataegus sanguinea, achieving the highest shoot proliferation rate.